ASTM E2149 PDF

ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period.

Log in Log out Edit. Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.

Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time.

Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously. Work Item s – proposed revisions of this standard. All the samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period.

The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension. This website stores cookies on your computer. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E – Microbe Investigations (MIS)

e149 It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. Test Package Request Form. Organisms typically used in this method: Record and report presence of solution activity. To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required. The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls.

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Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard.

This test method is designed to evaluate the resistance of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions. If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration.

Summary of Test Method: One-hour contact time in a buffer solution allows for metabolic stasis in the population. This dynamic shake flask test asfm developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

Link to Active This link will always route to the current Active version of the standard.

ASTM E2149 Antimicrobial Activity

These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

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We use this information to improve and customize your browsing experience and for analytics and metrics about our visitors — both on this website and other media. To find out more about the cookies we use, see our Privacy Policy. Work Item s – proposed revisions of this standard. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension.

No regulated reduction limits currently exist for general antimicrobial claims made using this method. This standard does wstm purport to address all of the safety concerns, if any, associated with its use.

ASTM E – Method Determining the Antimicrobial Activity

This asym shake flash test was developed for routine quality control and screening test. The flask is placed onto a wrist-action shaker and shaken for a desired exposure time, typically 1 hour. Maria Curry ext Wrist Action Shaker. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors. No other units of measurement are included in this standard. The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions.

A single cookie will be used in your browser to remember your preference not to be tracked. This standard does not purport to address all of the safety concerns, if any, associated with its use.

Link to Active This link will always route to the current Active version of the standard. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.